Year: 2013 | Month: November | Volume 6 | Issue 4s
Photodynamic Potential Of Curcumin Nanoparticle With Chitosan On Adherent Canine Tumour Cell Line A72
Abstract: <div>Cancer is a highly incomprehensible and complex disease. Current treatment strategies are associated with adverse toxicities like multidrug resistance by cancer cells, destruction of normal cells, hair loss etc. The present work was aimed to use the natural photo sensitizer curcumin from C. longa and C.aromatica in nanoscale along with chitosan for the Photo Dynamic Therapy of cancer using adherent canine tumour cell line A72.Curcumin was extracted from C. longa and C.aromatica with water, acetone and ethanol. Light Source used for PDT was 12V Philips Endura LED 7W MR16 dimmable lamp. Photo sensitizers and nano materials were characterized by AFM and UV-Vis spectrum. Photosensitizers from 50µg/50µl to 0.393µg/50µl was used for toxicity assay . Toxicity of all photosensitizers were evaluated in darkness and light by MTT assay. Acetone extraction of C.longa yielded comparatively more curcumin than ethanol and water. Yield of curcumin was 0.25%, 0.16and 0.05% in acetone, ethanol and water respectively . UV-Vis absorption spectrum of curcumin showed peak at 425 nm. In darkness the toxicity of curcumin to A72 was 9% and curcumin nano particle was 11% at a concentration of 50 µg/50µl .Cellular toxicity by light alone was found to be 2%.</div>
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